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Global mapping of ZBTB7A transcription factor binding sites in HepG2 cells

Cellular & Molecular Biology Letters volume 15, pages 260–271 (2010)Cite this article

Abstract

ZBTB7A is a known proto-oncogene that is implicated in carcinogenesis and cell differentiation and development. Fully understanding the function of ZBTB7A in cellular processes could provide useful strategies for cancer treatment and development-associated disease therapy. Here, global mapping of ZBTB7A transcription factor binding sites was developed by utilizing microarray technology in HepG2 cells. The data obtained from the microarrays was further validated via chromatin immunoprecipitation-PCR (ChIP-PCR) and real time-PCR, and it was revealed that ZBTB7A may be one of the regulators of neural development. ZBTB7A target signal pathways were identified in signal pathway and GO (Gene Ontology) analyses. This is the first report on the global mapping of ZBTB7A downstream direct targets, and these findings will be useful in understanding the roles of ZBTB7A in cellular processes.

Abbreviations

ChIP:

chromatin immunoprecipitation

FASN:

synthase gene

GO:

gene ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

PMSF:

phenylmethylsulfonyl fluoride

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Authors and Affiliations

  1. The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, 518055, People’s Republic of China

    Xuyu Zu, Lingling Yu, Jing Tian, Feng Liu, Qinsheng Sun, Shengnan He, Guang Sun, Weishi Luo & Yuyang Jiang

  2. School of Medicine, Tsinghua University, Beijing, 100084, People’s Republic of China

    Yuyang Jiang

  3. Medical Systems Biology Research Center, Tsinghua University, Beijing, 100084, People’s Republic of China

    Yiming Sun

  4. National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway, Beijing, 102206, People’s Republic of China

    Xuyu Zu, Lingling Yu, Yiming Sun, Jing Tian, Feng Liu, Qinsheng Sun, Shengnan He, Guang Sun, Weishi Luo & Yuyang Jiang

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  1. Xuyu Zu
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  2. Lingling Yu
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  8. Guang Sun
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Correspondence to Yuyang Jiang.

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Zu, X., Yu, L., Sun, Y. et al. Global mapping of ZBTB7A transcription factor binding sites in HepG2 cells. Cell Mol Biol Lett 15, 260–271 (2010). https://doiorg.publicaciones.saludcastillayleon.es/10.2478/s11658-010-0003-7

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  • DOI: https://doiorg.publicaciones.saludcastillayleon.es/10.2478/s11658-010-0003-7

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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