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Fig. 6  | Cellular & Molecular Biology Letters

Fig. 6 

From: Multi-omics integration reveals Vha68-3 as a testicular aging-specific factor that coordinates spermatid elongation through mitochondrial metabolic homeostasis

Fig. 6 

Identification of Vha68-3 binding proteins. A Pull-down assay for Vha68-3 binding proteins. B Number of Vha68-3 binding proteins identified via LC–MS/MS. C Ten representative Vha68-3 binding proteins. D Three-dimensional structural models for Vha68-3 and Muc proteins. E Visualization of the docking interface for the Vha68-3/Muc complex. F Reciprocal co-IP of Vha68-3 and Muc proteins. G PDH activity in control (n = 4) and Vha68-3 KO (n = 4) testes at 40 days. Bars represent mean ± SEM; Student’s t-test; ***P < 0.001

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