Fig. 6

The NF-κB–HIF-1α signal promotes neutrophil infiltration by promoting fibroblast secretion of CXCL1 through glycolysis. A, B Western blot diagrams of COL1A1, α-SMA, and CXCL1 after 2-DG treatment of PSCs. C mRNA levels of IL-6, IL-1β, TNF-α, and CXCL1 after 2-DG treatment of PSCs. D Fluorescence image of significantly reduced α-SMA after 2-DG treatment of PSCs. E Fluorescence image of significantly reduced ki-67 after 2-DG treatment of PSCs. F Transwell co-culture chamber: PSCs are cultured in the lower chamber with or without 2-DG added, neutrophils are cultured in the upper chamber, and the number of neutrophils in the lower chamber is observed after 24 h (neutrophils are labeled with MPO, green). G Effect of transfection of PSCs with si-P65 or OE-HIF-1α on extracellular acidification rate curve (ECAR). H Effect of transfection of PSCs with si-P65 and OE-HIF-1α on basal glycolytic capacity. I Effect of transfection of PSCs with si-P65 and OE-HIF-1α on maximum glycolytic capacity. J, K Protein levels of COL1A1, α-SMA, and CXCL1 after transfection of PSCs with si-P65 or OE-HIF-1α. L mRNA expression levels of IL-6, IL-1β, TNF-α, and CXCL1 after transfection of PSCs with si-P65 and OE-HIF-1α. M Migration images after transfection of PSCs with si-P65 and OE-HIF-1α. N Clone formation images after transfection of PSCs with si-P65 and OE-HIF-1α. O Secretion level of CXCL1 after transfection of PSCs with si-P65 and OE-HIF-1α. Student’s t-test was used for comparison between two groups. *P < 0.05 is considered statistically significant, **P < 0.01, ***P < 0.001