Fig. 5

NF-κB promotes HIF-1α accumulation by inhibiting PHD2, thereby enhancing PSC activation and CXCL1 secretion. A Verification of the expression level of P65 mRNA after PSCs are transfected with si-P65. B Verification of the protein expression level of P65 after PSCs are transfected with si-P65. C Observation of the mRNA level of HIF-1α after PSCs are transfected with si-P65. D Western blot diagram of P65 and HIF-1α after PSCs are transfected with si-P65. E Expression level of HIF-1α in the nucleus after PSCs are transfected with si-P65. F Verification of the expression level of HIF-1α mRNA after PSCs are transfected with si-HIF-1α. G Verification of the protein expression level of HIF-1α after PSCs are transfected with si-HIF-1α. H–I Western blot diagram of NF-κB signaling molecules, α-SMA, and CXCL1 after PSCs are transfected with si-HIF-1α. J MG-132 inhibits the ubiquitin–proteasome system, as shown by the effect of si-P65 on HIF-1α ubiquitination. An IP experiment was performed on HIF-1α to verify the ubiquitin level of each group by Western blot. The expression of P65 and HIF-1α in total protein was verified by a Western blot experiment. K mRNA expression level of PHD2 after PSCs are transfected with si-P65. L Western blot diagram of PHD2 after PSCs are transfected with HIF-1α. Student’s t-test was used for comparison between two groups. *P < 0.05 is considered statistically significant, **P < 0.01, ***P < 0.001