Fig. 3

The infiltration of neutrophils in acute pancreatitis is related to the activation of fibroblasts. A Heat map of marker expression of fibroblast subgroups. B T-SNE diagram of fibroblast subgroups. C Proportion diagram of fibroblast subgroups in normal control group and AP group. Pseudotemporal analysis diagram of fibroblast subgroups: D the pseudo-temporal direction diagram, with colors from deep to light, E the intergroup pseudotemporal diagram where fibroblasts differentiate from the control group to the AP group, and F the fibroblast subgroup differentiation diagram. G Relative expression levels of activation markers α-SMA, COL1A1, and VIMENTIN in each subgroup. H Relative expression level of CXCL1 in each subgroup. I Intercellular communication analysis: ligand–receptor pair. J CXCL1 is expressed in fibroblasts, and CXCR2 is expressed in neutrophils. K Scanning electron microscope image of neutrophils in interstitial fibers. L Immunofluorescence image, neutrophils (MPO, yellow), fibroblasts (COL1A1, green), nucleus (DAPI, blue). Student’s t-test was used for comparison between two groups. *P < 0.05 is considered statistically significant, **P < 0.01, ***P < 0.001