Fig. 2

scRNA-seq indicates that neutrophils in pancreatic tissue show heterogeneity during AP. A Cell atlas of pancreatic tissues of mice in normal control group and AP group. B Marker genes that distinguish various types of cells. C Heat map of marker gene expression of neutrophil subgroups. D Cell atlas of neutrophil subgroups. E Proportion of neutrophil subgroups in normal control group and AP group. F Multicolor immunofluorescence confirmed the existence of CD177 + neutrophil subgroup, CD54 + neutrophil subgroup, Siglec-F + neutrophil subgroup, neutrophils (MPO, yellow), CD177 is light blue, CD54 is red, Siglec-F is orange, and the nucleus is dark blue in pancreatic tissues of mice with AP. G Pseudotemporal analysis diagram of three neutrophil subgroups. H–J Expression trends of markers of three neutrophil subgroups on the time axis. K Gene dynamics analysis of neutrophils. The results show that there are three expression patterns of highly variable genes in neutrophils. L Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of highly variable genes in each module. Student’s t-test was used for comparison between two groups. *P < 0.05 is considered statistically significant, **P < 0.01, ***P < 0.001