Fig. 7

YY1 transcriptionally activates USP13 and forms a carcinogenic YY1/USP13 axis in HCC. A The transcriptional factors that regulate USP13 were predicted by PROMO, AliBaba2.1, Animal TFDB, and GeneCards. B, C WB and qRT-PCR results of USP13 expression after knockdown or overexpression of YY1. D ChIP-qPCR analyzed the binding motifs of USP13 promoter with YY1 protein in MHCC97H cells. E The binding motif 3 of the USP13 promoter was mutated, and the relative luciferase activity from wild-type and mutant-type-transfected cells was measured by a dual luciferase reporter system. F WWP1 proteins were detected by WB with overexpression of USP13 in YY1-knockdown HCC cells. G WWP1 proteins were detected by WB with knockdown of USP13 in YY1-overexpression HCC cells. H Representative IHC staining image of YY1, USP13 and WWP1 in HCC tissue were displayed. I The correlation between YY1 and USP13 were analyzed based on IHC score. J The correlation between USP13 and WWP1 was analyzed on the basis of IHC score. K The correlation of between YY1 and WWP1 was analyzed according to IHC score. Scale bars: 50 µm