Fig. 5

USP13 regulates HCC cell behaviors via WWP1. A WB assay detected the Akt/mTOR pathway-related protein expression levels with WWP1 overexpression in USP13-knockdown HCC cells. B WB assay detected the Akt/mTOR pathway-related protein expression levels with WWP1-knockdown in USP13-overexpression HCC cells. C CCK-8 and (D) colony formation assays were used to detect cell proliferation ability. E Spheroid formation assays were used to detect cell stemness ability. F, G Wound healing and (H, I) Transwell assays were used to detect cell migration ability. Scale bars: 275 µm