Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: YY1 induced USP13 transcriptional activation drives the malignant progression of hepatocellular carcinoma by deubiquitinating WWP1

Fig. 4

USP13 removed the K29- and K48-linked polyubiquitination chain of WWP1. A WB results of WWP1 expression upon USP13 knockdown in HCC cells. B WB results of WWP1 expression upon USP13 overexpression in HCC cells. C, D The ubiquitination of WWP1 was analyzed in HCC cells with stable USP13 knockdown or overexpression. E WWP1 protein level was detected in HCC cells treated with CHX and MG132 or HCQ. F CHX assays were conducted to detect the half-life of WWP1 in Huh7 shUSP13 and shNC cells. G Quantitative analysis of WWP1 protein expression by ImageJ. H, I WB results of the ubiquitination of WWP1 in HEK293T cells with co-transfection of Myc-WWP1, HA-ubiquitin, or mutations, and either the empty vectors or Flag-USP13

Back to article page

Cellular & Molecular Biology Letters

ISSN: 1689-1392

Contact us