Fig. 3

USP13 interacted with WWP1 in HCC cells. A Co-IP assay detected the endogenous interaction between USP13 and WWP1 with a USP13 antibody in HCC cells and HEK293T cells. B Co-IP results with a WWP1 antibody in HCC cells and HEK293T cells. C Co-IP assays showed the exogenous interaction between USP13 and WWP1 with a Flag antibody. D Co-IP results of the binding between USP13 and WWP1 with a Myc antibody. E The interaction between USP13 truncation mutants and WWP1. F Immunofluorescence indicated the colocalization of USP13 and WWP1 in Huh7 and MHCC97H cells. Scale bars: 50 µm