Fig. 2

USP13 was associated with HCC cell proliferation, migration, and stemness. A, B Huh7 and MHCC97H were infected with shNC, shUSP13-1, and shUSP13-2 lentivirus. qRT-PCR and WB were used to determine the mRNA and protein level of USP13. C, D CCK-8 and (E) colony formation assays were used to detect cell proliferation ability. F Transwell and (G) wound healing assays were used to detect cell migrate ability. H Spheroid formation assay was used to detect cell stemness. I Several stemness-related proteins expression was detected. Scale bars: 275 µm