Fig. 7

p21 knockdown restores differentiation capacity in ceramide-treated myoblasts. A, B Cdkn1a silencing significantly restored the ceramide-induced downregulation of MHC (type I; slow) expression (n = 3). C, D The ceramide-induced inhibition of myocyte differentiation was significantly restored by Cdkn1a silencing (n = 5). E Cdkn1a knockdown significantly inhibited the ceramide-induced upregulation of senescence-associated secretory cytokines, IL-6 and TNF-α (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired t-test. MHC, myosin heavy chain; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha