Fig. 1

Tolerogenic DCs of transgenic NOD.Stat5b-CA mice express higher USP7 than immunogenic DCs of NOD mice. A Representative FACS plots showing gating strategy used for USP7 expression analysis in purified splenic CD11c + DCs from the spleen. B Counter plots showing CD11c⁺USP7⁺ cell frequencies in purified splenic DCs of NOD and transgenic NOD.Stat5b-CA mice. C Representative bar graph showing USP7 frequency. D Absolute numbers of USP7 expressing DCs. E Histogram showing USP7 expression level in purified splenic CD11c⁺ DCs. F Mean fluorescence intensity (MFI) of USP7 expression and (G) Western blot analysis of USP7 expression and β-actin used as a loading control. Data are shown as the mean ± SEM of three independent experiments. The two-tailed unpaired Student’s t-test was used. *P < 0.05; **P < 0.01.****P < 0.0001