Fig. 7

ILC2s are enriched in PCa tissues. A Consecutive sections of HG PCa tissue were double stained for IL-33 and GATA3 (representative samples depicted) or for IL-33 and CD3. Arrows indicate tumor areas with immune foci containing ILC2, recognized as GATA3⁺ CD3⁻ lymphocytes. Scale bar: 1000 μm. B Magnified view of the area marked by the red arrow showing the indicated double stainings on consecutive sections. GATA3⁺ CD3⁻ lymphocytes are visible in the center of the immune foci. Scale bar: 50 μm. C–H Analysis of single-cell RNA sequencing (scRNA-seq) data from a publicly available PCa dataset. C UMAP plot highlighting ILC2, defined as PTPRC⁺GATA3⁺ CD3⁻ (blue dots). D Comparison of the abundance (% of total cells) of ILC2s in high-grade (HG) versus low-grade (LG) PCa tissues. E UMAP plot showing IL33-expressing cells, with a blue–yellow scale indicating IL-33 RNA expression levels. F Quantification of IL-33-expressing cells in HG and LG PCa tissues. G UMAP plot showing IL-18-expressing cells, with a blue–yellow scale indicating IL-18 RNA expression levels. H Quantification of IL18-expressing cells in HG and LG PCa tissues