Fig. 6

ILC2-derived IL-13 promotes PCa cell migration and invasion. A, B Prostate cancer cell line mRNA expression of IL13RA1 (A) and IL13RA2 (B) receptors via GEDS platform. C Representative example of a wound healing assay (20× magnification) performed using PC3 cancer cells after incubation with ILC2 CM or ILC2 CM + anti-IL-13 blocking antibody. D Quantification of the healed wound area at 24 and 48 h. E, F Representative example (E) and quantification (F) of clonogenic assays performed using PC3 cancer cells after incubation with ILC2 CM or ILC2 CM + anti-IL-13 blocking antibody for 10 days. G, H Expression of MMP9 (G) and MMP2 (H) assessed by qPCR analysis in PC3 cancer cells upon incubation with ILC2 CM or ILC2 CM + anti-IL-13 blocking antibody. Data shown as mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001) and analyzed by Wilcoxon and/or one-way ANOVA tests