Fig. 7

CD155 activates Wnt/β-catenin signaling via the PI3K-AKT pathway. A The volcano plot illustrates the increase and decrease in the number of genes in 143B cells following CD155 knockdown, in comparison with control cells. B KEGG enrichment analysis revealed that the PI3K-AKT signaling pathway exhibited the most significant alteration. C Western blot was utilized to assess alterations in the protein levels of phosphorylated AKT and phosphorylated GSK3β in OS cells with CD155 knockdown. D Proteins potentially interacting with CD155 (PVR) predicted by the STRING database. E, F The interaction between CD155 and SRC in SJSA-1 and 143B cells was examined through a co-IP experiment. G Western blot was utilized to assess alterations in the protein levels of phosphorylated SRC in OS cells following the knockdown of CD155