Fig. 6
From: Troxerutin suppresses the stemness of osteosarcoma via the CD155/SRC/β-catenin signaling axis
CD155 facilitates OS malignancy via Wnt/β-catenin activation. A The nuclear translocation of β-Ccatenin in OS cells with knocked-down CD155 was evaluated using the western blot following the addition of HLY78. (B–D) The CCK-8 (B) assay and colony formation assay (C, D) were employed to assess the proliferative capacity of OS cells upon the addition of HLY78 to cells with downregulated CD155 expression. E, F Utilizing the Transwell assay, the invasion and migration capacities of OS cells were evaluated. G, H The CCK-8 assay was employed to assess the resistance of OS cells toward cisplatin. I Representative images of the in vitro sphere formation assay. J The quantity of spheres in the in vitro sphere formation assay. K, L The activity of ALDH in OS cells was measured using fluorescence-activated cell sorting analysis. Representative images and quantitative data of ALDH activity results are presented. *p < 0.05; **p < 0.01; ***p < 0.001