Fig. 5
From: Troxerutin suppresses the stemness of osteosarcoma via the CD155/SRC/β-catenin signaling axis
β-Catenin promotes malignant phenotypes in OS in vitro. A Western blot is used to assess the levels of β-catenin protein in SJSA-1 and 143B cells following the knock-down of CTNNB1. B–D The CCK-8 assay (B) and colony formation assay (C, D) were employed to assess the proliferative capacity of OS cells following CTNNB1 knockdown. E, F Utilizing the Transwell assay, the invasion and migration capacities of OS cells were evaluated. G, H The CCK-8 assay was employed to assess the resistance of OS cells towards cisplatin. I Representative images of in vitro sphere formation assay conducted on control cells and CTNNB1-knockdown OS cells. J The quantity of spheres in the in vitro sphere formation assay conducted on CTNNB1-knockdown OS cells and their respective control cells. K, L The activity of ALDH in CTNNB1-knockdown OS cells was measured using fluorescence-activated cell sorting analysis. Representative images and quantitative data of ALDH activity results are presented. *p < 0.05; **p < 0.01; ***p < 0.001