Fig. 4

Overexpression of CD155 augments malignant OS phenotypes. A Western blot analysis was employed to assess the levels of CD155 protein in U2OS, SJSA-1, and 143B cells following overexpression of CD155. B–D CCK-8 assay (B) and colony formation assay (C, D) were employed to assess the proliferative capacity of OS cells following the overexpression of CD155. E, F The Transwell assay was used to assess the invasive and migratory capabilities of OS cells. G, H The CCK-8 assay was employed to assess the resistance of OS cells toward cisplatin. I qRT-PCR was utilized to assess the expression levels of stemness markers in U2OS, SJSA-1, and 143B cells following the overexpression of CD155. J Representative images of in vitro sphere formation assays conducted on control cells and CD155-overexpressing OS cells. K The quantity of sphere in the in vitro sphere formation assay conducted on CD155-overexpressing OS cells and their respective control cells. L, M The activity of ALDH in CD155-overexpressing OS cells was measured using fluorescence-activated cell sorting analysis. Representative images and quantitative data of ALDH activity results are presented. N Detection of alterations in β-catenin nuclear protein levels in OS cells following the overexpression of CD155 via western blotting. *p < 0.05; **p < 0.01; ***p < 0.001