Fig. 2

CD155 enhances malignant phenotypes of OS. A Western blot is used to assess the levels of CD155 protein in SJSA-1 and 143B cells following the knockdown of CD155. B–D The CCK-8 assay (B) and colony formation assay (C, D) to assess the proliferative capacity of OS cells following CD155 knockdown. E, F Utilizing the Transwell assay, the invasion and migration capacities of OS cells were evaluated. G, H The CCK-8 assay was employed to assess the resistance of OS cells toward cisplatin. I, J Flow cytometry was employed to assess the apoptotic state of OS cells following knockdown of CD155. K, L Flow cytometry was utilized to assess the apoptotic status of OS cells following cisplatin treatment (SJSA-1 cells pretreated with 9 μM cisplatin for 48 h and 143B cells pretreated with 1 μM cisplatin for 48 h). M Knocking down CD155 can effectively suppress the growth of 143B cells in nude mice. N After a period of 27 days, the tumor were extracted and subsequently weighed. O The tumor volume was monitored every 3 days to generate a tumor growth curve accordingly. P Western blot experiments were performed to assess the expression level of CD155 in 143B cells following tumor formation in nude mice, followed by grayscale analysis. Q IHC staining of tumor sections was performed using CD155 and KI-67 antibodies. *p < 0.05; **p < 0.01; ***p < 0.001