Fig. 8

Liver IRI is protected in vivo by MAFF via the CLCF1/STAT3 pathway. A Hepatic IRI model. B and F Histological analysis using H&E staining revealed the presence of necrotic areas in the liver tissue (n = 5); scale bars = 100 μm and 20 μm. C and G IHC was conducted to evaluate P-STAT3 expression in liver sections (n = 5); scale bars = 100 μm and 20 μm. D and H TUNEL staining and statistical analysis were performed on ischemic liver sections obtained from the mice; scale bar = 50 μm. E and I Immunofluorescence staining was used to identify inflammatory cells expressing CD11b (red) in liver slices affected by ischemia (n = 5); scale bar = 100 μm. J and K ALT and AST levels in the blood were assessed in the rAd-NC, rAd-shMAFF, and rAd-shMAFF + CLCF1 groups (n = 5)