Fig. 7

MAFF binding to BACH1 facilitates the activation of the CLCF1/STAT3 signaling pathway. A–E Following immunoprecipitation (IP) with MAFF antibody or IgG (negative control antibody), samples collected from hepatocytes were subjected to electrophoresis. Subsequently, Coomassie brilliant blue staining was performed. This staining technique was employed after the samples were subjected to H/R. The present study employed mass spectrum analysis to examine IP-MAFF and IP-IgG lysates. A Venn diagram was generated, and GO analysis was performed. F Interactions among 50 proteins. G Co-IP of MAFF and BACH1 in hepatocytes after H/R. H Immunofluorescence staining of MAFF, BACH1, and DAPI in hepatocytes after H/R; scale bar = 20 μm. I RT-qPCR was used to assess CLCF1 levels (n = 3). J The effect of BACH1 on the STAT3 pathway in liver cells was evaluated using WB analysis after suppressing BACH1 expression. K A luciferase test was conducted utilizing the pGL4-luciferase vector. The transcriptional activity of the discovered MARE was reduced upon BACH1 or MAFF knockdown (n = 3)