Fig. 6
From: MAFF alleviates hepatic ischemia–reperfusion injury by regulating the CLCF1/STAT3 signaling pathway
Hepatocyte apoptosis is regulated by the MAFF-mediated CLCF1 pathway. A–B Flow cytometry was used to evaluate cell apoptosis in hepatocytes exposed to shCLCF1 (n = 3). C–D The impact of CLCF1 on AML12 cell apoptosis both with and without MAFF knockdown, as determined using flow cytometry (n = 3). E WB was used to assess STAT3 activation in AML12 cells subjected to either normoxia or H/R in the shNC and shMAFF groups. F WB was used to assess STAT3 activation in AML12 cells subjected to either normoxia or H/R in the shNC group and shCLCF1 group. G WB was used to assess STAT3 activation in AML12 cells under H/R stimulation following the addition of exogenous CLCF1 in the shNC and shMAFF groups