Fig. 5
From: MAFF alleviates hepatic ischemia–reperfusion injury by regulating the CLCF1/STAT3 signaling pathway
Identification of MAFF target genes using genome-wide analysis. A Volcano plot showing gene expression after interference with MAFF (│logFC│ > 1, p < 0.05). B Volcano plot showing gene expression after MAFF overexpression (│logFC│ > 1, p < 0.05). C The motif algorithm was used to identify the MAFF-binding motif on the basis of MARE. D Heatmap showing that MAFF-binding sites are located mainly in the promoter region. E Chromosomal coordinates of MAFF-binding sites. F Integration of RNA sequencing, CUT&Tag methods and MRG to identify genes under the control of MAFF genes during IRI. G RT-qPCR was used to assess CLCF1 mRNA expression levels following IRI. H The CUT&Tag sequencing results revealed an abundance of DNA sequence tags. I ChIP-PCR showed that MAFF binds to CLCF1