Fig. 4

MAFF relieves inflammation in hepatic IRI. A At 6 h after hepatic IRI, the liver tissues of both rAd-NC and rAd-shMAFF mice were analyzed to assess mRNA levels of inflammatory cytokines and chemokines (n = 3). B and C Immunofluorescence was performed to detect CD11b-positive inflammatory cells (red) in ischemic liver sections obtained from mice in the specified experimental groups (n = 6); scale bar = 50 and 100 μm. D and E IHC was performed to assess Ly6G expression in ischemic liver sections obtained from mice in the specified experimental groups (n = 6); scale bar = 100 μm. F At 6 h after hepatic IRI, the liver tissues of both the rAd-NC and the rAd-OEMAFF mice were analyzed to assess mRNA levels of inflammatory cytokines and chemokines (n = 3). G and H Immunofluorescence staining was used to identify inflammatory cells expressing CD11b (red) in liver sections from the mice assigned to the designated experimental groups (n = 6); scale bars = 50 and 100 μm. I and J IHC was conducted to evaluate the presence of Ly6G in liver sections affected by ischemia, using samples obtained from mice assigned to specific experimental groups (n = 6); scale bar = 100 μm