Fig. 3

MAFF protects against liver damage. A Hepatic IRI model. B Expression of MAFF protein in the livers of rAd-NC group and rAd-shMAFF group. C The serum levels of ALT and AST were identified in both rAd-NC and rAd-shMAFF mice at 6 h after hepatic IRI (n = 5). D and E At 6 h post hepatic IRI, liver tissue samples from mice treated with rAd-NC and rAd-shMAFF were analyzed using H&E staining (n = 5); scale bar = 100 μm. F and G TUNEL staining was used to evaluated cell death of ischemic liver sections (n = 5); scale bar = 50 μm. N The expression of cleaved-caspase-3, Bax, and Bcl2 in the livers of both rAd-NC and rAd-shMAFF mice was analyzed using WB. H Expression of MAFF protein in the hepatic tissues of mice administered with rAd-NC and rAd-OEMAFF. I ALT and AST were identified in both rAd-NC and rAd-OEMAFF mice after hepatic IRI (n = 5). J and K At 6 h after hepatic IR, H&E staining demonstrated necrotic regions of liver tissue from rAd-NC and rAd-OEMAFF mice (n = 5); scale bar = 100 μm. L and M TUNEL staining was performed on ischemic liver slices obtained from mice belonging to the specified groups (n = 5); scale bar = 50 μm. The expression of cleaved-caspase-3, Bax, and Bcl2 in the livers of both rAd-NC and rAd-OEMAFF mice was analyzed using WB