Fig. 1

IRI-related genes were identified by sequencing and database analysis after modeling. A H&E staining was used to evaluate areas of necrosis; scale bar = 20 and 100 μm. B TUNEL staining was performed to detect cell death; scale bar = 50 μm. C and D Heatmap and volcano map showing the differentially expressed genes (DEGs) identified after IRI (│logFC│ > 1, p < 0.05). E and F GO and KEGG analyses of the differentially expressed genes. G Abundance map of the top 200 DEGs according to the sequencing results. H Abundance map of the DEGs in the GSE14951 dataset (|logFC│ > 2, p < 0.05). I Abundance map of the DEGs in the GSE151648 dataset (|logFC│ > 2, p < 0.05). J The Venn diagram illustrates the overlap between the sequencing screening and analysis of liver transplant databases