Fig. 5
From: A novel mechanism for A-to-I RNA-edited CYP1A1 in promoting cancer progression in NSCLC
CYP1A1 overediting activated the PI3K/AKT signaling pathways in NSCLC cells. A Heatmap illustrating the signature of the top 50 genes upregulated by A549I462V as determined by transcriptome profile analysis. FDR p < 0.01. B KEGG pathway enrichment analyses of DEGs between A549 and A549I462V cells. C GSEA of the A549I462V targeted upregulated gene signature. D Western blot of total Akt in the serum samples from normal control subjects and patients with lung cancer with CYP1A1 overediting or not. β-Actin was used as the internal control. The corresponding quantification data are shown in E. F Western blot showing expression level of total Akt and phospho-Akt in both A549 and H1299 cells after transfection with different types of CYP1A1. G Western blot analysis of total Akt and phospho-Akt in A549 and A549I462V cells. Values above each band represent band intensity and were calculated using ImageJ software. Band intensity in the first lane was normalized as 1.0. One-way ANOVA with Tukey’s test as post hoc test was used to assess the difference; *p < 0.05; **p < 0.01; ***p < 0.001