Fig. 7

Combination of circNF1 blockade and anti-PD-L1 therapy exhibits favorable antitumor activity in vivo. A Schematic diagram of the experimental design of TE1 tumor-bearing humanized mouse model. B Macroscopic image of excised ESCC tumors from indicated groups. C, D The tumor weights (C) and growth curves (D) of tumor volume after initial implantation in each group. E, F Representative IHC images (E) and statistical quantitation (F) of immunostaining of ANXA1, PD-L1, Ki67, cleaved Caspase-3, CD8, TNF-α, IFN-γ, and GZMB in xenograft tumor tissues. Corresponding 1–4 groups are si-NC+iso, si-circNF1+iso, si-NC+αPD-L1, and si-circNF1+αPD-L1, respectively. Scale bar: 500 μm. The data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001