Fig. 6

Identification of CHD8 target genes whose expression is regulated by CHD8 direct binding-mediated chromatin remodeling. A Overlapping between genes bound by CHD8 and genes upregulated or downregulated in Chd8^−/− cells identified through RNA-seq. RNA-seq Cutoff: Log2FC = 0.7. B Enriched pathways of CHD8 direct targets revealed by overlapping CHIP-seq data with RNA-seq data. Gene set enrichment was carried out by using Enrichr. C Heatmaps of RNA-seq, CHIP-seq, and ATAC-seq of CHD8 direct targets that may contribute to Chd8^−/− Treg phenotypes. D ATAC-seq and anti-CHD8 CHIP-seq tracks of Cdkn1a, Bhlhe40, and Perp in WT and/or Chd8^−/− Tregs