Fig. 5

CHD8 deficiency leads to chromatin remodeling. Splenic CD4⁺ T cells were isolated from WT and Chd8^−/− mice by AutoMACS. YFP⁺ cells were then sorted by FACS and analyzed by ATAC-seq and CHIP-seq. A Heatmap of ATAC-seq peaks in WT and Chd8^−/− Tregs showing ± 1.5 kb around the ATAC-seq peak center. Results are from one WT male mouse, one Chd8^−/− male mouse, and one Chd8^−/− female mouse. Cutoff: Log2FC = 0.4. B Genes with altered ATAC-seq peaks in Chd8^−/− versus WT cells were analyzed for enrichment in hallmark pathways. Upregulated hallmark pathways and leading-edge genes are shown. Note: no downregulated hallmark pathways were found. C Heatmap of anti-CHD8 CHIP-seq peaks compared with IgG control in Tregs. Results are from one WT male mouse and one WT female mouse. D GSEA enrichment plot of representative pathway of CHD8 binding genes. E Heatmap of ATAC up and ATAC down peaks at CHD8 binding sites in Chd8^−/− Tregs. F Heatmap of ATAC up and ATAC down peaks at non-CHD8 binding sites in Chd8^−/− Tregs