Fig. 4
From: Genetic and epigenetic regulation of Treg cell fitness by autism-related chromatin remodeler CHD8

CHD8 deficiency alters global gene expression. Splenic CD4+ T cells were isolated from WT and Chd8−/− mice (WT: one male + one female; Chd8−/−: one male + one female) by AutoMACS. YFP+ cells were then sorted by FACS and analyzed for gene expression profiles by RNA-Seq. A Heatmap of differentially expressed genes between WT and Chd8−/− Tregs. B Representative upregulated hallmark pathways in Chd8−/− Tregs. C Representative GSEA enrichment plots of upregulated pathways in Chd8−/− Tregs. D Volcano plot of differentially expressed genes between WT and Chd8−/− Tregs. Log2FC cutoff: 1.0; FDR < 0.05. E Fold changes (Chd8−/− versus WT) of representative genes in mTORC1, p53, and ROS signaling pathways