Fig. 6

N-glycan expression significantly increases in the thalamus following SNL. A ROI of thalamus. B–I Hierarchical clustering heatmap, PLS-DA plot, volcano plot, and MALDI mass spectrometry images with intensity plot of the ipsilateral (B–E) or contralateral (F–I) thalamus. The correlation of the top 25 N-glycans detected in three samples from the sham (green) and SNL (red) groups is shown and presented in the hierarchical clustering heatmap (H: hexose, N: N-acetylglucosamine, and F: fucose). PLS-DA plot of N-glycans of the sham and SNL groups with 95% confidence region. In the volcano plots, p < 0.05 was considered statistically significant. Representative MALDI mass spectrometry images and intensity plots of N-glycans show significant differences between the sham and SNL groups (unpaired t-test, **p < 0.01, ***p < 0.001, error bar: SEM). Data are representative of n = 3 independent experiments. Scale bars = 2 mm