Fig. 2

Intervention of CDC20 in DOX-induced myocardial cell damage. a Results of GO and KEGG analyses on the differentially expressed genes between the control group and DOX treatment; b Volcano plot illustrating gene expression and CDC20 expression; c Expression of CDC20 and β-actin using western blotting (n = 4); d Immunofluorescence staining to detect CDC20 expression in control group and DOX (1 and 2 μM) of H9C2 cells (n = 3, bar = 50 μm); e Quantified expression of CDC20 in H9C2 cells using qPCR in control group and DOX (2 μM) (n = 4); f Expression of CDC20 in the hearts of mice using qPCR (n = 4); g Expression of CDC20 in NRCMs cells using qPCR (n = 3); h Caspase-3 activity in each experimental group (n = 4); i Expression of Bax, Bcl-2, and β-actin using western blotting (n = 3). DOX: doxorubicin, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes, qPCR: quantitative polymerase chain reaction. Data presented as mean ± SD. Statistical analysis performed with one-way ANOVA or two-way ANOVA