Fig. 1

Doxorubicin-induced cardiac injury and apoptosis. a The cellular morphological changes in control group and DOX (2 μM) group were observed under a microscope (n = 3, bar = 50 μm); b Caspase 3 activity in control group and DOX (2 μM) (n = 4); c Weight change of mice in control group and DOX (20 mg/kg) (n = 6); d Representative image of cardiac function in control group and DOX (20 mg/kg) (n = 4); e EF% statistics of mice in control group and DOX (20 mg/kg) (n = 4); f HW/TL statistics of mice in control group and DOX (20 mg/kg) (n = 4); g–j Measured expression levels of Bax, Bcl-2, and β-actin in H9C2, AC16, NRCM at 2 μM concentration of DOX, and mouse hearts at 20 mg/kg concentration of DOX (n = 3). EF: ejection factor, HW/TL: heart weight/tibia length. Data presented as mean ± SD. Statistical analysis performed with Student’s t test