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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Novel regulation mechanism of histone methyltransferase SMYD5 in rheumatoid arthritis

Fig. 4

SMYD5 promotes inflammatory response in IL-1β-induced FLS. A–C SMYD5 was knocked down using SMYD5 siRNA in IL-1β (10 ng/ml, 24 h)-induced FLS, with control siRNA (siNC) as the negative control. A RT-qPCR analysis of Smyd5, Nos2, Ptgs2, and Il6 mRNA levels, n = 3. B Immunoblot analysis and quantification of SMYD5, iNOS, and COX2, with β-tubulin as a loading control, n = 5 or n = 6. C ELISA analysis for IL-6 in the supernatant from IL-1β induced FLS pretransfected with or without SMYD5 siRNA, n = 3. DF SMYD5 was overexpressed in FLS using lentivirus vectors encoding SMYD5 (LV-SMYD5). D Immunoblot analysis and quantification of SMYD5, iNOS, and COX2, with β-tubulin as a loading control, n = 4 or n = 6. E RT-qPCR analysis of Nos2, Ptgs2, and Il6 mRNA levels, n = 3. F ELISA for IL-6 and TNF-α levels in the supernatant of FLS with or without LV-SMYD5 treatment, n = 4. Data presented as mean ± SEM, p values calculated by two-tailed Student’s t-test (DF) or one-way ANOVA test (AC), *p < 0.05, ***p < 0.001

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