Fig. 1

Prominent expression of SMYD5 in synovial tissues of patients with RA and IL-1β treated FLS. A Heatmap showing the protein expression of histone methyltransferases in synovial tissues from patients with osteoarthritis (OA) and rheumatoid arthritis (RA). B Immunoblot analysis and quantification of SMYD5 in synovial tissues from patients with OA or RA, n = 3. C Immunofluorescence detection of SMYD5 in synovial tissues from patients with OA or RA. Scale bars, 100 μm. D–H Rat fibroblast-like synoviocytes (FLS) were treated with IL-1β (10 ng/ml) for indicated times. D Immunoblot analysis and quantification of inflammation markers (iNOS and COX2) and proliferation markers (Cyclin D1 and MMP9), n = 4. E mRNA levels of Nos2, Ptgs2, Il6, and Ccnd1 measured by RT-qPCR, n = 3. F Representative images of EdU staining in IL-1β treated or untreated FLS. Scale bars, 50 μm. Quantification of EdU-positive cells in each group is shown in the right panel, n = 6. The protein expression (G) and mRNA level (H) of SMYD5 were detected by immunoblots and RT-qPCR, respectively, n = 3. Quantification of SMYD5 protein expression in G normalized to β-tubulin. Data presented as mean ± SEM, p values calculated by two-tailed Student’s t-test (B, F) or one-way ANOVA test (D, E, G, H), *p < 0.05, **p < 0.01, ***p < 0.001, n.s. means no significance