Fig. 5

CRKL regulated trophoblast proliferation, migration, invasion, and apoptosis by activating the C3G/Rap1 signaling pathway. A HTR-8/SVneo cells were transfected with sh-C3G or sh-NC, and the knockdown efficiency of C3G was verified by Western blot after 48 h. HTR-8/SVneo cells were co-transfected with Oe-NC or Oe-CRKL and sh-NC or sh-C3G. B Rap1-GTP activity was detected using pull-down assay after 48 h of transfection. C CCK-8 assay was performed to evaluate transfected HTR-8/SVneo cell proliferation at 0, 24, 48, and 72 h. D Transwell assay was performed to evaluate transfected HTR-8/SVneo cell migration and invasion after 48 h. E Flow cytometry was performed to evaluate transfected HTR-8/SVneo cell apoptosis after 48 h. Data presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001