Fig. 4

tRF-5028c regulated trophoblast proliferation, migration, invasion, and apoptosis by inhibiting CRKL expression. HTR-8/SVneo cells were co-transfected with inhibitor NC or tRF-5028c inhibitor and sh-NC or sh-CRKL. A, B The mRNA and protein levels of CRKL in transfected HTR-8/SVneo cells were assessed by qRT-PCR and western blot after 48 h. C CCK-8 assay was performed to evaluate transfected HTR-8/SVneo cell proliferation at 0, 24, 48, and 72 h. D Transwell assay was performed to evaluate transfected HTR-8/SVneo cell migration and invasion after 48 h. E Flow cytometry was performed to evaluate transfected HTR-8/SVneo cell apoptosis after 48 h. F Rap1-GTP activity was detected using pull-down assay in transfected HTR-8/SVneo cell after 48 h. Data presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001