Fig. 2

tRF-5028c promoted trophoblast proliferation, migration, and invasion and inhibited apoptosis. A tRF-5028c location in HTR-8/SVneo cells was analyzed by FISH assay. HTR-8/SVneo cells were transfected with tRF-5028c inhibitor/mimic or inhibitor/mimic NC. B, C The transfection efficiencies were detected by fluorescence microscopy and qRT-PCR after 24 h. D CCK-8 assay was performed to evaluate transfected HTR-8/SVneo cell proliferation at 0, 24, 48, and 72 h. E Transwell assay was performed to evaluate transfected HTR-8/SVneo cell migration and invasion after 48 h. F Flow cytometry was performed to evaluate transfected HTR-8/SVneo cell apoptosis after 48 h. Data presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001