Fig. 7
From: Repression of ZNFX1 by LncRNA ZFAS1 mediates tobacco-induced pulmonary carcinogenesis
CSC-mediated activation of ZFAS1 coincides with epigenetic alterations in the promoter of ZNFX1. A qChIP analysis of DNMT3A, DNMT3B, and DNMT1 levels within the first CpG island of the ZNFX1 promoter in SAEC and Calu-6 cells exposed to NM or CSC, following overexpression or knockdown of ZFAS1. B qChIP analysis of EZH2, SUZ12, and BMI1 levels within the promoter of ZNFX1 in SAEC and Calu-6 cells either exposed to NM or CSC following overexpression or knockdown of ZFAS1. C qChIP analysis of H3K4me3 or H3K27me3 within the promoter of ZNFX1 in SAECs and Calu-6 cells exposed to NM or CSC following overexpression or knockdown of ZFAS1. CSC and ZFAS1 decrease levels of H3K4me3 while increasing H3K27me3 levels in the proximal promoter region (0 to −1 kb) of ZNFX1 in SAEC and Calu-6 cells; depletion of ZFAS1 partially abrogates CSC-induced alterations in these histone marks within this region. Data are mean ± SEM; T-test; n = 3; * p < 0.05; **p < 0.01