Fig. 11

CSC upregulates ZFAS1 via selective interaction of NFkB with the ZFAS1 enhancer. A qRT-PCR analysis demonstrating expression of NFkB-p65 in normal respiratory epithelia and lung cancer cells cultured in the presence or absence of CSC. B qRT-PCR analysis demonstrating that siRNA knockdown of NFkB-p65 significantly decreased ZFAS1 expression in normal respiratory epithelia and lung cancer cells treated with or without exposure to CSC. C/D) Quantitative ChIP analysis demonstrating increased levels of NFkB in the enhancer region of ZFAS1 in SAEC and Calu-6 cells following CSC exposure (C) or in lung cancer tissues from both smokers and nonsmokers (D). E qRT-PCR analysis demonstrating ZFAS1 and ZNFX1 expression in A549 lung cancer cells treated with or without mythysticin at 0 to 1000 nM concentration for 48 h. Methysticin dose-dependently enhanced expression of ZNFX1 while dose-dependently inhibiting expression of ZFAS1 in normal respiratory epithelia and lung cancer cells. Data are mean ± SEM; T-test; n = 3; * p < 0.05; **p < 0.01