Fig. 5

Expression of KLF5 in wound tissue and the verification of the targeted regulation relationship between KLF5 and CXCL12. A Histological analysis of KLF5 in wound tissue in patients with diabetic ulcers and diabetic mice. × 100, Scale bar = 50 μm; ×200, Scale bar = 100 μm. B Immunofluorescence diagram of KLF5 in the wound tissue of diabetic mice on day 14 post-wounding; dashed line is the boundary between epidermis and dermis; E is the epidermis and D is the dermis. Scale bar = 50 μm. C Targeted modulation was measured by luciferase reporter gene assays. The ratios of Firefly luciferase (FLU) activity to Renilla luciferase (RLU) activity are displayed. D Western blot analysis of KLF5 protein expression in each group of ADSCs. E Western blot quantitative analysis of KLF5 expression level. F ELISA detection of CXCL12 secretion in the culture supernatant of ADSCs. All experiments were performed in triplicate and were repeated three times to confirm the findings (*p < 0.05). Data represent mean ± SD, *p < 0.05, ** p < 0.01, ns, not significant