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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: KLF5 enhances CXCL12 transcription in adipose-derived stem cells to promote endothelial progenitor cells neovascularization and accelerate diabetic wound healing

Fig. 2

Characterization of ADSCs and verification of CXCL12 knockdown. A Normal cultured ADSCs under a microscope, scale bar = 100 μm; B ADSCs after adipogenic differentiation, scale bar = 100 μm. C ADSCs after chondrogenic differentiation, scale bar = 100 μm. D ADSCs after osteogenic differentiation, scale bar = 100 μm. E Flow cytometry analysis of ADSC surface markers: CD45, CD34, CD29, and CD90. F Western blot analysis of CXCL12 protein expression in each group of ADSCs. G Western blot quantitative analysis of CXCL12 expression level. H RT-qPCR detection of CXCL12 mRNA expression in each group of ADSCs. I ELISA detection of CXCL12 secretion in the culture supernatant of ADSCs. All experiments were performed in triplicate and were repeated three times to confirm the findings (*p < 0.05). Data are presented as mean ± standard deviation (SD). *p < 0.05, ***p < 0.001, ****p < 0.0001 versus shNC; #p < 0.05 versus shRNA3

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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