Fig. 8

hUSC-Exo inhibited CTX-induced apoptosis of GCs by activating the PI3K/AKT/mTOR signal pathway. A, B The expressions of the proteins related to the PI3K/Akt/mTOR signaling pathway, such as P-mTOR, P-PI3K ,and P-AKT, were detected by Western blot analysis in GCs in the treatment of hUSC-Exo. C, D The expressions of the proteins related to the PI3K/Akt/mTOR signaling pathway were determined by Western blot analysis in GCs in the treatment of hUSC-Exo with or without LY294002, an inhibitor of the PI3K/AKT/mTOR signaling pathway. E The apoptotic ratios of GCs in the normal group, CTX group, CTX + hUSC-Exo group, and CTX + hUSC-Exo + LY294002 group were detected by flow cytometry. F The quantitative results were obtained from the flow cytometry (E) *P < 0.05, **P < 0.01