Fig. 7

miR-27b-3p inhibited the outflux of intracellular serine by selectively suppressing the expressions of SLC1A4 in CTX-induced POF. A The expressions of Slc1a4, Tmem86a, Pdia4, Ankrd1, and Heg1 were detected in GCs by qRT-PCR. B Predicted target sites of Slc1a4 3′UTR (red letters show the predicted pairing of the target region and miRNA). C Schematic diagram of SLC1A4 and serine transmembrane transport. D, E Detection of knockdown efficiency of Slc1a4 on GCs after siRNA transfection by qPCR and Western blotting. F The relative intensity of Slc1a4 in Western blotting. G Live–dead staining was used to detect death in the normal + NC group, CTX + NC group, CTX + siRNA group, and CTX + serine group. H The apoptotic ratios of GCs in each group were detected by flow cytometry. I The apoptosis of GCs was quantitatively detected by flow cytometry *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001