Fig. 5

hUSC inhibited CTX-induced GC apoptosis through miRNA-221-3p and miR-27b-3p in hUSC-Exo. A Volcano plot of differential expression miRNAs in the hUSC-Exo and DFL-Exo groups, n = 3. B Heatmap of the differentially expressed miRNAs between hUSC-Exo and DFL-Exo (red: increased expression; blue: decreased expression). C High expression of miR-27b-3p and miR-221-3p in hUSC-Exo was confirmed using qPCR. D miR-221-3p and miR-27b-3p expression of GCs was elevated after transient overexpression of miR-221-3p and miR-27b-3p. E The apoptotic ratios of GCs in the normal + NC group, CTX + NC group, CTX + miR-221-3p mimic group, and CTX + miR-27b-3p mimic group were detected by flow cytometry. F Live–dead staining to identify apoptosis of GCs was conducted using immunofluorescence staining *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001