Fig. 6

Fer-1 alleviates blue light-induced retinal degeneration and RPE cell ferroptosis in Abca4^−/−Rdh8^−/− mice; 3-month-old Abca4^−/−Rdh8^−/− mice were injected intraperitoneally with DMSO or Fer-1 (4 mg/kg). A total of 1 h later, the mice were irradiated by blue light for 30 min after their pupils were dilated, followed by once daily administration of DMSO or Fer-1 for additional 4 days. A Full-field ERG was utilized to detect retinal function under scotopic conditions. The a- and b-wave amplitudes of scotopic ERG were measured at an intensity of 10 cd s/m² by using a computer-based system (n = 6). Alternatively, the a- and b-wave amplitudes of scotopic ERG were measured at serial intensities of 0.01, 0.1, 1, and 10 cd s/m² (n = 6). B Fundus imaging. C ZO-1 staining of the RPE/choroid. D OCT imaging (n = 6). E HE staining (n = 6). F Western blots of SLC7A11 and GPX4 (n = 3). G Lipid peroxidation in the RPE/choroid (n = 3)