Fig. 5

Ferroptosis involves blue light-induced retinal degeneration in Abca4^−/−Rdh8^−/− mice. C57BL/6 J and Abca4^−/−Rdh8^−/− mice at 3 months of age were exposed to 430 nm blue light for 30 min and then dark adapted for 5 days. Control C57BL/6 J and Abca4^−/−Rdh8^−/− mice were normally raised for 5 days in the dark without exposure to blue light. A Full-field ERG was used to evaluate mouse retinal function under scotopic conditions. The a- and b-wave amplitudes of scotopic ERG were measured at an intensity of 10 cd s/m² by using a computer-based system (n = 6). Alternatively, the a- and b-wave amplitudes of scotopic ERG were measured at serial intensities of 0.01, 0.1, 1, and 10 cd s/m² (n = 6). B Fundus imaging. C ZO-1 staining of the RPE/choroid. D OCT imaging (n = 6). E HE staining. ONL thickness measurement on the temporal-nasal meridian (n = 6). F Western blots of SLC7A11 and GPX4 (n = 3). G Lipid peroxidation in the RPE/choroid (n = 3). RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars, 20 μm