Fig. 4
From: Exposure of A2E to blue light promotes ferroptosis in the retinal pigment epithelium
Ferroptosis inhibitor Fer-1 mitigates blue light-induced ferroptosis in A2E-loaded ARPE-19 cells. A Cell viability (n = 6). ARPE-19 cells that were incubated with 25 μM A2E for 48 h were pretreated with serial concentrations of Fer-1 (20, 30 and 40 μM) for 2 h and then illuminated by blue light for 30 min, followed by 24 h of incubation. B−E ARPE-19 cells that were treated for 48 h with 25 μM A2E were preincubated with 30 μM Fer-1 for 2 h and exposed for 30 min to blue light, followed by 24 h of incubation. B LDH release detection (n = 6). C H2DCFDA staining (n = 3). Scale bars, 20 μm. D Lipid peroxidation (n = 3). Scale bars, 10 μm. E Western blots of SLC7A11 and GPX4 (n = 3)